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KMID : 0387720120230030210
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Korean Journal of Blood Transfusion
2012 Volume.23 No. 3 p.210 ~ p.216
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Analysis for Human Immunodeficiency Virus 1 Subtype in Korean Blood Donors
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Cho Youn-Jung
Kim Myung-Han
Kwon So-Yong
Cho Nam-Sun
Youn Kyoung-Won
Jee Yong-Hun
Choi Kyoung-Young
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Abstract
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Background: Genetic variants of virus appear to differ depending on the country, race, infection route, and so
on. To characterize the main HIV subtype in infected blood donors and inquire about the route of HIV infection,
we analyzed HIV subtype for samples that showed reactive results on the anti-HIV 1/2 and HIV-1 NAT test from
September 2007 to February 2010.
Methods: To identify the HIV-1 subtype of the 90 samples that showed reactive results on the anti-HIV test and
HIV-1 NAT, we performed HIV 1/2 Western blot assay, HIV RNA quantitative assay, HIV-1 nested PCR, and
HIV-1 RNA sequencing.
Results: A total of 85 samples (94.4%) were confirmed to be HIV-1 subtypes. Among them, 82 samples (96.5%)
were subtype B; and subtype A, C, and G was confirmed for one case each (1.2% for each case). We could
not identify the subtype of the other five samples. One of them was amplified by nested PCR, but was not confirmed
of the subtype, and four samples were not amplified even by nested PCR.
Conclusion: The main HIV-1 subtype among the HIV-infected blood donors was confirmed to be subtype B.
In addition, we identified one case each of HIV-1 subtype A, C, and G, which was not detected in blood donors
in the past. It appeared that the route of HIV infection in Korea had become complicated. Therefore, we concluded
that continuous research for HIV subtype analysis and efficient management of blood donors is needed.
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KEYWORD
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Blood donor, HIV-1 subtype, Infection route
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